Anti-Phospho-p38 MAPK (Thr180,Tyr182) MAPK14 Antibody

Product Name

Anti-Phospho-p38 MAPK (Thr180,Tyr182) MAPK14 Antibody

View all p38 alpha Antibodies

SKU/Catalog Number

P00176

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-p38 MAPK (Thr180, Tyr182) MAPK14 Antibody (Catalog # P00176). Tested in WB, IHC applications. This antibody reacts with Human, Mouse.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-p38 MAPK (Thr180,Tyr182) MAPK14 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00176)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Clone Number

JE44-80

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr180/Tyr182 of rat p38/MAPK, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P00176 is reactive to MAPK14 in Human, Mouse

Applications

P00176 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

41.293kDa

Background of p38 alpha

The three Mitogen-Activated Protein Kinases (MAPKs) are evolutionarily conserved protein kinases that control a vast array of cellular processes. p38 MAPK is one of these kinases and it is activated by both inflammatory cytokines and by stress (Johnson and Lapadat, 2002; Shi and Gaestel, 2002). The p38 MAPK is thought to be particularly important in diseases like asthma and autoimmunity but it also plays important roles in the stress response of the nervous system (Philip and Armstead, 2003; Ying et al., 2002). Like the other MAPKs, p38 is activated by a dual specificity kinase that phosphorylates Thr-180 and Tyr-182 (Lin et al., 1995).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000
IHC: 1:250

Validation Images & Assay Conditions

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Immunostaining of human breast cancer tissue showing p38 when phosphorylated at Thr¹⁸⁰/Tyr¹⁸².

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Western blot of HeLa cell lysates that had been treated with UV (~254nm) for 0' or 30' showing the specific immunolabeling of the ~39 kDa p38 MAPK protein phosphorylated at Thr¹⁸⁰/Tyr¹⁸².

Gene/Protein Information For MAPK14 (Source: Uniprot.org, NCBI)

Alternative Names

MAPK14; p38 alpha; SAPK2a MAPK14 CSBP, CSBP1, CSBP2, CSPB1, EXIP, Mxi2, PRKM14, PRKM15, RK, SAPK2A, p38, p38ALPHA mitogen-activated protein kinase 14 mitogen-activated protein kinase 14|CSAID-binding protein|MAP kinase 14|MAP kinase Mxi2|MAP kinase p38 alpha|MAX-interacting protein 2|cytokine suppressive anti-inflammatory drug binding protein|mitogen-activated protein kinase p38 alpha|p38 MAP kinase|p38 mitogen activated protein kinase|p38alpha Exip|stress-activated protein kinase 2A

For more info on MAPK14, check out the MAPK14 Infographic

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In this infographic, you will see the following information for MAPK14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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